Journal: Cancer Science

Article Title: CCL2 induces resistance to the antiproliferative effect of cabazitaxel in prostate cancer cells

doi: 10.1111/cas.13876

Figure Lengend Snippet: The CCR2 antagonist restored the sensitivity to cabazitaxel in vivo. A, After 2 wk of acclimatization, 2 × 10 6 DU145 cells were implanted subcutaneously in SCID mice. The control group was intraperitoneally injected with 20 μ L of DMSO, and the cabazitaxel group was intraperitoneally injected with cabazitaxel weekly (days 0, 7 and 14) at a dose of 7 mg/kg diluted with 20 μ L of DMSO (n = 5). The tumor size was measured every other day using a caliper. B, Body weight was measured every other day using a scale. C, After 2 wk of acclimatization, 2 × 10 6 DU145‐TxR/CxR cells were implanted subcutaneously in SCID mice. The following groups were compared: control, cabazitaxel alone, CCR2 antagonist alone, and a combination of cabazitaxel and CCR2 antagonist. The control group was injected with 20 μ L of DMSO. Cabazitaxel was injected weekly (days 0, 7 and 14) at a dose of 7 mg/kg, and the CCR2 antagonist was injected every other day at a dose of 50 μ g/kg (n = 6). The left, middle and right bars on the right side of the graph illustrate the comparison between the combination group and the CCR2 antagonist group, the cabazitaxel group and the control group, respectively. The tumor size was measured every other day using a caliper. D, On day 21, the mice were killed and the tumors extracted. E, Body weight was measured every other day using a scale. Data are shown as means ± SEM

Article Snippet: Intact male SCID mice (aged 6‐7 weeks) were obtained from CLEA Japan (Tokyo, Japan).

Techniques: In Vivo, Control, Injection, Comparison

Journal: iScience

Article Title: Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity

doi: 10.1016/j.isci.2023.105962

Figure Lengend Snippet:

Article Snippet: To generate xenograft tumors of CRC organoid lines, 2 × 10 3 organoids were suspended in a 1:1 mixture of medium and Matrigel (Corning, 354234) and subcutaneously injected into the flank of NOD/Scid mice (4-5 weeks old) (CLEA Japan).

Techniques: Recombinant, Concentration Assay, Cell Viability Assay, SYBR Green Assay, Expressing, Microarray, Derivative Assay, Software

Journal: Cancer Science

Article Title: Establishment and molecular profiling of a novel human pancreatic cancer panel for 5‐FU

doi: 10.1111/j.1349-7006.2008.00896.x

Figure Lengend Snippet: Phase‐contrast micrographs of the 10 established cell lines in this study (Sui65‐Sui74) at the 25–30th passage (upper column). Original magnification, ×200. Histological section of a tumor established by s.c. injection of one of the cell lines into a SCID mouse (lower column). HE Stain, ×400.

Article Snippet: Female SCID mice (C.B.17/Icr Jcl‐scid) were purchased from CLEA Japan (Tokyo, Japan), and maintained under specific‐pathogen‐free conditions.

Techniques: Injection, H&E Stain

Journal: Cancer Science

Article Title: Establishment and molecular profiling of a novel human pancreatic cancer panel for 5‐FU

doi: 10.1111/j.1349-7006.2008.00896.x

Figure Lengend Snippet: Effect of 5‐fluorouracil (5‐FU) on Sui70 and Sui72 tumor growth in the SCID mouse. Six mice from each group were sacrificed when moribund, or on Day 28 or 40. The tumor mass was measured at predetermined time intervals in two dimensions with calipers, and the tumor volume was calculated according to the equation (l × w2)/2 [l = length, w = width].( 18 ) Pancreatic carcinoma was confirmed by histopathology.

Article Snippet: Female SCID mice (C.B.17/Icr Jcl‐scid) were purchased from CLEA Japan (Tokyo, Japan), and maintained under specific‐pathogen‐free conditions.

Techniques: Histopathology

Journal: Cancers

Article Title: RNF149 Promotes HCC Progression through Its E3 Ubiquitin Ligase Activity

doi: 10.3390/cancers15215203

Figure Lengend Snippet: RNF149 promotes subcutaneous tumorigenesis in mice. ( A ) Subcutaneous tumor formation assays were performed in NOD-SCID mice using Huh7 cell lines stably overexpressing RNF149. Representative pictures are shown. ( B ) Protein expression of RNF149 in the recovered tumors was detected by WB, and the representative images of No. 1, No. 4, and No. 6 mice are displayed. ( C , D ) Tumor size and weight were determined ( n = 6). * p < 0.05. The uncropped bolts are shown in .

Article Snippet: Male NOD-SCID mice (SpePharm Biotechnology, Beijing, China), aged 4 weeks, were housed in a special pathogen-free animal facility.

Techniques: Stable Transfection, Expressing

Journal: bioRxiv

Article Title: Human iPSC-derived hypertrophic chondrocytes reveal a mutation-specific unfolded protein response in chondrodysplasias

doi: 10.1101/2020.05.19.103960

Figure Lengend Snippet: (A-C) Histology of growth plate-like structures from tissue collected on day 56 after transplantation into immunodeficient mice. (top) Safranin O staining, (middle) von Kossa staining, (bottom) immunostaining. Scale bars, 200 μm. Similar results were observed in two or more growth plate-like structures for each clone. (D) von Kossa staining of mutant (top) or isogenic control (bottom) pellets transplanted on day 28 of HI and collected after 20 days in vivo . Similar results were obtained in six pellets transplanted into three different mice (two pellets per mouse). Scale bar, 1 mm. (E) Quantification of von Kossa-positive areas. Values are expressed as mean±SEM. Results are calculated as n=3 from three different mice with two pellets each. (n.s., no significant difference by unpaired two-sided t-test).

Article Snippet: This cell suspension was mixed 1:1 with matrigel (BD) and 100 μl were injected subcutaneously into a minimum of six male immunodeficient NOD/ShiJic-scid Jcl (NOD-SCID) mice (CLEA Japan).

Techniques: Transplantation Assay, Staining, Immunostaining, Mutagenesis, In Vivo